Complete Biosynthesis of Adipic Acid in Pseudomonas Putida

Summary: “The goal of my research project is to develop a novel biosynthesis pathway in Pseudomonas putida to convert waste lignin into adipic acid. The proposed process can theoretically produce adipic acid while emitting 60-80% less CO2 equivalents and consuming 20% less energy than conventional methods. My work explores the use of enoate reductase enzymes to synthesize adipic acid from the endogenous muconate pathway, with future aims including enzyme engineering and host development using genetic interventions.”